Strategic Deployment of Rotenone: Mechanistic Insights an...
2025-10-21
Rotenone, a gold-standard mitochondrial Complex I inhibitor, is transforming how translational researchers interrogate mitochondrial dysfunction, apoptosis, and neurodegenerative disease mechanisms. This thought-leadership article goes beyond the basics—integrating new findings on mitochondrial proteostasis (including the post-translational regulation of OGDH by TCAIM), offering actionable strategies for experimental design, and providing a roadmap for advancing clinical and preclinical models. By contextualizing rotenone's unique mechanistic profile and experimental advantages, this piece empowers researchers to accelerate discovery in mitochondrial biology and disease.